aav 2 9 Search Results


adeno-associated virus aav2/9-camkii-ichloc-2a-tdimer2  (Eppendorf AG)
90
Eppendorf AG adeno-associated virus aav2/9-camkii-ichloc-2a-tdimer2
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Adeno Associated Virus Aav2/9 Camkii Ichloc 2a Tdimer2, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav2/9-cmv-dio-erbb4-shrna-egfp  (SunBio Inc)
90
SunBio Inc aav2/9-cmv-dio-erbb4-shrna-egfp
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Aav2/9 Cmv Dio Erbb4 Shrna Egfp, supplied by SunBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav8- control  (Shanghai Genechem Ltd)
90
Shanghai Genechem Ltd aav8- control
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Aav8 Control, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav2  (Nature Biotechnology)
90
Nature Biotechnology aav2
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Aav2, supplied by Nature Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adeno-associated virus (aav) targeting to knockdown the entpd1 gene by shrna-entpd1 via cre-loxp strategy  (BrainVTA (Wuhan) Co Ltd)
90
BrainVTA (Wuhan) Co Ltd adeno-associated virus (aav) targeting to knockdown the entpd1 gene by shrna-entpd1 via cre-loxp strategy
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Adeno Associated Virus (Aav) Targeting To Knockdown The Entpd1 Gene By Shrna Entpd1 Via Cre Loxp Strategy, supplied by BrainVTA (Wuhan) Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adeno-associated virus aav2/9 serotype with ef1a promoter  (NGM Biopharmaceuticals)
90
NGM Biopharmaceuticals adeno-associated virus aav2/9 serotype with ef1a promoter
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Adeno Associated Virus Aav2/9 Serotype With Ef1a Promoter, supplied by NGM Biopharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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poros-aav2/9 elution pool  (BIA Separations)
90
BIA Separations poros-aav2/9 elution pool
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Poros Aav2/9 Elution Pool, supplied by BIA Separations, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav2/9  (Hoefer)
90
Hoefer aav2/9
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Aav2/9, supplied by Hoefer, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav2/9-cmv bgi-cre-egfp-pa  (Shanghai Model Organisms Center)
90
Shanghai Model Organisms Center aav2/9-cmv bgi-cre-egfp-pa
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Aav2/9 Cmv Bgi Cre Egfp Pa, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav2/9-camkii-tdtomato  (Cold Spring Harbor Laboratory Meetings)
90
Cold Spring Harbor Laboratory Meetings aav2/9-camkii-tdtomato
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Aav2/9 Camkii Tdtomato, supplied by Cold Spring Harbor Laboratory Meetings, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav2/9–camkiiα–gcamp6s virus  (Micropump Inc)
90
Micropump Inc aav2/9–camkiiα–gcamp6s virus
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Aav2/9–Camkiiα–Gcamp6s Virus, supplied by Micropump Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aav2/9–camkiiα–gcamp6s virus/product/Micropump Inc
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aav2/9-camkii-shwnt7a-egfp  (GL Biochem)
90
GL Biochem aav2/9-camkii-shwnt7a-egfp
( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed <t>tdimer2</t> was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.
Aav2/9 Camkii Shwnt7a Egfp, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed tdimer2 was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.

Journal: Scientific Reports

Article Title: An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo

doi: 10.1038/srep14807

Figure Lengend Snippet: ( A ) Overview of neuronal morphology 5 days after electroporation (maximum intensity projection of two-photon images). ( B) Fluorescence of co-expressed tdimer2 was used to target transfected neurons for electrophysiological recordings (same cells as in ( A )). (C) Dodt contrast image of cells shown in ( B ). (D) Photocurrents in response to a single light pulse (476 nm, 5 ms, 1 mW/mm 2 ) at different holding potentials. Photocurrents reversed at very negative holding potentials and were large at depolarized holding potentials, where the Cl − inward driving force was highest (same neuron as in ( A – C )). The inset shows the onset of the photocurrents at higher temporal resolution. Indicated holding potentials were rounded to full numbers after subtraction of the liquid junction potential (−10.6 mV). Indicated tau value was derived from 9 independent measurements in 9 slice cultures.

Article Snippet: An adeno-associated virus AAV2/9-CamKII-iChloC-2A-tdimer2 was produced by Ingke Braren and Kristin Bobsin at the HEXT core facility of the University Medical Center Hamburg-Eppendorf.

Techniques: Electroporation, Fluorescence, Transfection, Derivative Assay